IGEM:Caltech/2007/Project/N: Difference between revisions
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==Original Goals== | ==Original Goals== | ||
The objective of this subproject project was to determine the necessary concentration of N protein required for lysis. The N protein is active in the early stages of viral development, even before a choice is made between lysis and lysogeny, so in the absence of this protein the virus will not be infectious. | The objective of this subproject project was to determine the necessary concentration of N protein required for lysis. The N protein is active in the early stages of viral development, even before a choice is made between lysis and lysogeny, so in the absence of this protein the virus will not be infectious. | ||
The constructed system consists of the N gene driven by the Ptet promoter and followed by a transcription terminator (B0015). This device is followed by a sequence of either a strong or medium strength promoter (J23100 and J23116 respectively), TetR gene and terminator, which is responsible for expressing TetR protein, which is a repressor that acts on the Ptet promoter. | |||
==Status and Future Plans== | ==Status and Future Plans== |