IGEM:American University/2009/Notebook/AU Quantum Dot Lab/2014/09/15: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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==Entry title==
==Objective==
* Insert your content here.
*Begin new synthesis of HgS NCs
 
==Protocol==
# In a round bottom flask 5mL of 5mM HgNO3
# 5mL BSA solution in MQ water and stir
# Correct the pH to approximately 9 using 1M NaOH
# Allow solution to stir overnight (12-18 hours)
# Add 4mL of 20mM Na2S and stir for 15 minutes
 
==Notes==
*Solutions for this synthesis were prepared by James C. Schwabacher
*The HgNO3 was still difficult to fully dissolve in solution and therefore it was sonicated until almost homogenous to be used
*The color of the bio-conjugates was light grey and will be monitored as it should be pale yellow after stirring.  




Latest revision as of 00:17, 27 September 2017

iGEM Project name 1 Main project page
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Objective

  • Begin new synthesis of HgS NCs

Protocol

  1. In a round bottom flask 5mL of 5mM HgNO3
  2. 5mL BSA solution in MQ water and stir
  3. Correct the pH to approximately 9 using 1M NaOH
  4. Allow solution to stir overnight (12-18 hours)
  5. Add 4mL of 20mM Na2S and stir for 15 minutes

Notes

  • Solutions for this synthesis were prepared by James C. Schwabacher
  • The HgNO3 was still difficult to fully dissolve in solution and therefore it was sonicated until almost homogenous to be used
  • The color of the bio-conjugates was light grey and will be monitored as it should be pale yellow after stirring.



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