IGEM:American University/2009/Notebook/AU Quantum Dot Lab/2014/09/15

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Objective

  • Begin new synthesis of HgS NCs

Protocol

  1. In a round bottom flask 5mL of 5mM HgNO3
  2. 5mL BSA solution in MQ water and stir
  3. Correct the pH to approximately 9 using 1M NaOH
  4. Allow solution to stir overnight (12-18 hours)
  5. Add 4mL of 20mM Na2S and stir for 15 minutes

Notes

  • Solutions for this synthesis were prepared by James C. Schwabacher
  • The HgNO3 was still difficult to fully dissolve in solution and therefore it was sonicated until almost homogenous to be used
  • The color of the bio-conjugates was light grey and will be monitored as it should be pale yellow after stirring.



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