Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/11/20: Difference between revisions

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Results from NanoDrop
Results from NanoDrop


Excel table
'''Excel table'''




Using Xba restriction enzyme which will preform 2 cuts- expending bands at 1934 bp and 4505 bp
Using Xba restriction enzyme which will preform 2 cuts- expending bands at 1934 bp and 4505 bp


insert picture
'''insert picture'''






plasmid validation template page
'''plasmid validation template page'''


KAH126 incluswa Hpdc, scar, mcherry: about 1000 bp
KAH126 incluswa Hpdc, scar, mcherry: about 1000 bp

Revision as of 16:06, 21 November 2015

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Baylee Murphy- 11/20/15

KAH126- MV2 Culture inoculation results from Session 2

  • Liquid cultures 1 and 2 both had cloudy yellow broth, indicating growth
  • Streaks for both showed colony growth

Samples for mini prep and analysis

  • (1) Sample 1- KAH126-MV2 colony 1, [BB#}; part = [length in bp]; vector= [length in bp]
  • (2) Sample 2- KAH126-MV2 colony 2, [BB#}; part = [length in bp]; vector= [length in bp]

Results from NanoDrop

Excel table


Using Xba restriction enzyme which will preform 2 cuts- expending bands at 1934 bp and 4505 bp

insert picture


plasmid validation template page

KAH126 incluswa Hpdc, scar, mcherry: about 1000 bp

  • forward primer- DD122 10 uM
  • reverse primer-DD123 10 uM


Samples sent for sequencing at Bio Design around 4:45pm

  • (1) Sample 1- KAH126-MV2 colony 1, forward primer DD122
  • (2) Sample 2- KAH126-MV2 colony 1, reverse primer DD123
  • (3) Sample 3- KAH126-MV2 colony 2, forward primer DD122
  • (4) Sample 4- KAH126-MV2 colony 2, reverse primer DD123






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