Haynes Lab:Notebook:Shared/Cloning Bootcamp
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This is the shared Notebook for Haynes Lab Cloning Bootcamp, a molecular biology training program organized by Dr. Karmella Haynes at Arizona State University.
STAGE A: Curating the Haynes Lab Plasmid DNA Collection
You have been assigned a plasmid or plasmids to “miniprep,” “verify,” and “submit to the Registry.” What does this mean and how do you get started? How do you finish? This guide will help you to complete your tasks, and learn about DNA and synthetic biology along the way. The purpose of curating the plasmid collection is to take some DNA from our freezer, produce a large batch of it, confirm that the reproduced DNA is not damaged, and to send that batch of DNA to a repository where the DNA will be shared with the world. This is a very important and educational task.
High-level concepts that you will learn about in greater detail include...
STAGE A consists of four Sessions: