Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/07/29: Difference between revisions

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{| {{table}}
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| Plasmid || OD260 || OD260/280 || ng/μL
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| 1. Plasmid 1 || --- || --- || ---
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| 2. Plasmid 2 || --- || --- || ---
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Revision as of 12:20, 29 July 2015

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Brady Dennison - 07/28/15

Sample 1: Liquid culture grew, but showed no signs of the mCherry gene. Plate had growth and showed the mCherry gene.

Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.

Sample 1 Nanodrop results:

.02 .02

.008 .01

2.453 1.977

20.031 20.325


Sample 2 Nanodrop results:

.187 .182

.098 .097

1.911 1.882

187.22 182.42

Plasmid Validation Template page

DNA Concentration Data


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'




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