Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/07/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab Cloning Bootcamp</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab Cloning Bootcamp</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene. | Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene. | ||
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| Plasmid || OD260 || OD260/280 || ng/μL | |||
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| 1. Plasmid 1 || .02 || 2.453 || 20.325 | |||
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| 2. Plasmid 2 || .187 || 1.911 || 187.22 | |||
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=Plasmid Validation Template page= | |||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = | | rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = 746, 2155 <br>2. Plasmid 2 = 746, 2155<br> | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:Bdbootcamp.jpeg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA(plasmid) || 3.0 μL | | DNA(plasmid) || 3.0 μL | ||
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* Order Number: | * Order Number: | ||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | <!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | ||
Latest revision as of 01:04, 27 September 2017
Haynes Lab Cloning Bootcamp | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||
Brady Dennison - 07/28/15Sample 1: Liquid culture grew, but showed no signs of the mCherry gene. Plate had growth and showed the mCherry gene. Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.
Plasmid Validation Template pageRestriction Digest Table
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