Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/27: Difference between revisions
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Treat samples in duplicate, for 12 samples in total. | Treat samples in duplicate, for 12 samples in total. | ||
==Notes== | |||
Culture density: 240,000 cells/mL * 5 mL culture = 1.2E6 cells total | |||
To make 1E7 cells/mL concentration, spin down and resuspend in 120 µL Resuspension Buffer R. | |||
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Revision as of 15:11, 27 May 2015
Neon transfection of K562 cell line | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
OverviewTrying transfection of K562 again, this time using the Neon transfection system. Protocol
Samples:
Treat samples in duplicate, for 12 samples in total. NotesCulture density: 240,000 cells/mL * 5 mL culture = 1.2E6 cells total To make 1E7 cells/mL concentration, spin down and resuspend in 120 µL Resuspension Buffer R.
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