Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/26

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K562 transfection optimization results Main project page
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Overview

Sample ID DNA (µg) LTX (µL) % transfection (avg) % transfection (st dev)
1422.81%0.004949747
2121.50%0.002404163
30.420.50%0.000636396
4631.90%0.003676955
51.531.86%0.000494975
60.630.77%0.000282843
7844.15%0.003676955
8241.52%0.003889087
90.841.12%0.000565685
101054.47%0.001626346
112.552.53%0.002192031
12152.53%0.013010765
0000.08%0.000494975

There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine.



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