Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/23: Difference between revisions
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==Goals== | ==Goals== | ||
* | * Harvest U2OS cells from 1st transfection attempt | ||
* Flow cytometry on cells to determine gene expression levels | |||
* <strike>RNA purification on cells to create cDNA library</strike> (see below, Results section)<br><br> | |||
==Protocol== | ==Protocol== |
Revision as of 15:06, 23 December 2014
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryToday we'll be harvesting the U2OS cells from the first transfection attempt. Fluorescence microscopy of the cells indicates there is little or no PcTF-RFP expression in the line, but we'll be doing a run through with flow cytometry and RNA purification regardless, as practice for future attempts. Goals
ProtocolMaterials
NotesObservations, measurements, placeholder info etc. go here Results/ConclusionsAny significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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