Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/23
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Today we'll be harvesting the U2OS cells from the first transfection attempt. Fluorescence microscopy of the cells indicates there is little or no PcTF-RFP expression in the line, but we'll be doing a run through with flow cytometry and RNA purification regardless, as practice for future attempts.
Overview of flow cytometry data:
Negative control does not have significantly different fluorescence values than the samples (data not shown).
Since the control has the same fluorescence levels as the samples (and therefore the same gene expression levels of PcTF-mCherry), we will not be performing the RNA extraction / purification today. Instead we'll be repeating this experiment tomorrow on the second set of transfected cells.