Haynes Lab:Notebook/Jan/2015/03/23: Difference between revisions
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*Benchling link: [https://benchling.com/hayneslab/f/RtoHDBrtPX-mammalian-basic-parts/seq-kD0Mzx2B-dpre/ DPRE] | *Benchling link: [https://benchling.com/hayneslab/f/RtoHDBrtPX-mammalian-basic-parts/seq-kD0Mzx2B-dpre/ DPRE] | ||
*Ampicillin resistance | *Ampicillin resistance | ||
*Length of plasmid: 1740 bp | *Length of plasmid: 1740 bp + 3200 bp V0120 backbone = 4940 bp | ||
*Transformation done on 3/23/2015 | *Transformation done on 3/23/2015 | ||
*Result for Plate 1: Medium growth over the plate. Lack of high growth may be related to plasmid's age (last cloned in 2011). Overall success. | *Result for Plate 1: Medium growth over the plate. Lack of high growth may be related to plasmid's age (last cloned in 2011). Overall success. | ||
Revision as of 20:27, 26 March 2015
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Transformation of DPRE/HoxD11.12
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