Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/28: Difference between revisions
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(Autocreate 2015/04/28 Entry for Haynes_Lab:Notebook/HPK-CFP_insertion_into_Gal4EED/Luc_using_CRISPR) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Transformation from DBN004_pSB1A3 Ligation</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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I'll be checking the plates for colonies and starting liquid cultures & plates from the samples if colonies appear. | |||
==Transformation Results== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Plate''' | |||
| align="center" style="background:#f0f0f0;"|'''# colonies''' | |||
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| neg. control||0 | |||
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| colony 1||10 | |||
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| colony 2||12 | |||
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| colony 5||4 | |||
|} | |||
==Liquid Cultures & Streaks== | |||
Four colonies were picked from each transformation plate. They're numbered 1.1, 1.2, 1.3, 1.4, 2.1 etc. where the first number is the original (source) colony and the second number is the colony picked today. There are twelve colonies picked in total, streaked on a library plate and placed in 5mL aliquots of LB + Amp. | |||
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Revision as of 15:12, 28 April 2015
 Transformation from DBN004_pSB1A3 Ligation
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OverviewI'll be checking the plates for colonies and starting liquid cultures & plates from the samples if colonies appear. Transformation Results
Liquid Cultures & StreaksFour colonies were picked from each transformation plate. They're numbered 1.1, 1.2, 1.3, 1.4, 2.1 etc. where the first number is the original (source) colony and the second number is the colony picked today. There are twelve colonies picked in total, streaked on a library plate and placed in 5mL aliquots of LB + Amp. | |||||||||||
