Haynes Lab:Notebook/Engineering PC-TFs/2015/01/26: Difference between revisions

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[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA[BL01: W2,W3*](CM9: W4,W5)|| [8.0 μL] (5.0 μL)
| DNA[BL05: W2,W3*](CM9: W4,W5)|| [8.0 μL] (5.0 μL)
|-
|-
| 10X buffer || 3.0 μL
| 10X buffer || 3.0 μL
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| dH<sub>2</sub>O || [17μL] (21μL)
| dH<sub>2</sub>O || [17μL] (21μL)
|-
|-
| Total || 15 μL --> 37°C/ 30 min.
| Total || 30 μL --> 37°C/ 30 min.
|}
|}
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Revision as of 12:00, 26 January 2015



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Summary

Ligation Digest and Gel Verification

Reagent Volume

Expected:
BL05 [XbaI/SpeI]
Forward insertion: 3659, 4064
Reverse insertion: 1538, 6185
No insertion: 1538, 4064

CMV/MV9 with [XbaI]
5197

 [[|400px|Today's gel]]

30μL/lane; 1% agarose;
Observed lengths:
W2 BL05 S1
W3 BL05 S2
W4 CMV/MV9 S1P(urple)
W5 CMV/MV9 S1B(lue)

Ladder

DNA[BL05: W2,W3*](CM9: W4,W5) [8.0 μL] (5.0 μL)
10X buffer 3.0 μL
XbaI 1.0 μL
SpeI 1.0 μL (0uL)
dH2O [17μL] (21μL)
Total 30 μL --> 37°C/ 30 min.




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