Haynes Lab:Notebook/Engineering PC-TFs/2014/12/02: Difference between revisions

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<u><b>Expected:</b></u>
<u><b>Expected:</b></u>
<br>
<br>
<br>BL01(Well: 4-8, 11-14), BL05(Well:2) <br>
CMV/MV9 (Well 1): 5200<br>
Forward insertion: 710, 7010. <br>
BL01 (Well 3): 2500, 3300<br>
Reverse insertion: 3230, 4490. <br>
BL05 (Well 5): 2500, 3300<br>
No insertion: 710, 4490. <br><br>


<u>CMV/MV9 [XbaI/NruI] (Well: 9)</u> <br>
710, 4490<br><br>




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<u>Observed lengths:</u> <br>
<u>Observed lengths:</u> <br>
W2(BL05Lig1): 5000, 700; <br>
 
W4(BL01Lig1): 3200, 4500; <br>
W5(BL01Lig2): 5000, 700; <br>
W6(BL01Lig3): 5000, 700; <br>
W7(BL01Lig4): 5000, 700; <br>
W8(BL01Lig5): 5000, 700; <br>
W9(Empty CMV/MV9): 5000, 700; <br>
W11(BL01Lig6): 5000, 700; <br>
W12(BL01Lig7): 5000, 700; <br>
W13(BL01Lig8): 5000, 700;<br>
W14(BL01Lig9): 5000, 700; <br>
|-
|-
| DNA(CMV/MV9, BL01, BL05) || 15.0 μL
| DNA(CMV/MV9, BL01, BL05) || 15.0 μL

Revision as of 16:41, 2 December 2014



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Summary

Reagent Volume

Expected:
CMV/MV9 (Well 1): 5200
BL01 (Well 3): 2500, 3300
BL05 (Well 5): 2500, 3300


 Today's gel

15 μL/lane; 1% agarose; Ladder

Observed lengths:

DNA(CMV/MV9, BL01, BL05) 15.0 μL
10X buffer 3.0 μL
XbaI 2.0 μL
SpeI (no SpeI for CMV/MV9) 2.0 μL
dH2O 8 (10 for CMV/MV9) μL
Total 30 μL --> 37°C/ 30 min.




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