Haynes Lab:Notebook/Engineering PC-TFs/2014/06/16: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 15: Line 15:
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br> 2520(insert)+4968(V0120 Vector) = 7488bp <br><u>Observed:</u><br> <br>
| rowspan="7" | <u>Expected:</u><br> 2520(insert)+4968(V0120 Vector) = 7488bp <br><u>Observed:</u><br> ~7000bp <br>
|-
|-
| DNA(BL05 M.P) || 8.0 μL
| DNA(BL05 M.P) || 8.0 μL
Line 33: Line 33:
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>2520(insert)+4968(V0120 Vector) = 7488bp <br><u>Observed:</u><br> <br>
| rowspan="7" | <u>Expected:</u><br>2520(insert)+4968(V0120 Vector) = 7488bp <br><u>Observed:</u><br> ~7000bp <br>
|-
|-
| DNA(BL05A) || 4.0 μL
| DNA(BL05A) || 4.0 μL
Line 51: Line 51:
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1431 (insert) + 4968 (V0120 Vector) = 6399bp <br><u>Observed:</u><br> <br>
| rowspan="7" | <u>Expected:</u><br>1431 (insert) + 4968 (V0120 Vector) = 6399bp <br><u>Observed:</u><br> ~4500 <br>
|-
|-
| DNA(BL09 M.P.) || 8.0 μL
| DNA(BL09 M.P.) || 8.0 μL
Line 69: Line 69:
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br> 1431 (insert) + 4968 (V0120 Vector) = 6399bp <br><u>Observed:</u><br> <br>
| rowspan="7" | <u>Expected:</u><br> 1431 (insert) + 4968 (V0120 Vector) = 6399bp <br><u>Observed:</u><br> ~4000 <br>
|-
|-
| DNA(BL09B) || 4.0 μL
| DNA(BL09B) || 4.0 μL
Line 86: Line 86:
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br> 5197bp <br><u>Observed:</u><br> <br>
| rowspan="7" | <u>Expected:</u><br> 5197bp <br><u>Observed:</u><br> ~4900 <br>
|-
|-
| DNA(Phosphorylated CMV/MV9 ) || 2.0 μL
| DNA(Phosphorylated CMV/MV9 ) || 2.0 μL
Line 108: Line 108:


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
 


__NOTOC__
__NOTOC__

Revision as of 13:37, 17 June 2014



 		<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

Digest All the Things Addendum

Well One: Ladder
Well Two

Reagent Volume Expected:
2520(insert)+4968(V0120 Vector) = 7488bp
Observed:
~7000bp
DNA(BL05 M.P) 8.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 4.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Three

Reagent Volume Expected:
2520(insert)+4968(V0120 Vector) = 7488bp
Observed:
~7000bp
DNA(BL05A) 4.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 8.5 μL
Total 15 μL --> 37°C/ 30 min.

Well Four

Reagent Volume Expected:
1431 (insert) + 4968 (V0120 Vector) = 6399bp
Observed:
~4500
DNA(BL09 M.P.) 8.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 4.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Five

Reagent Volume Expected:
1431 (insert) + 4968 (V0120 Vector) = 6399bp
Observed:
~4000
DNA(BL09B) 4.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 8.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Six

Reagent Volume Expected:
5197bp
Observed:
~4900
DNA(Phosphorylated CMV/MV9 ) 2.0 μL
10X buffer 1.5 μL
dH2O 11.5 μL
Total 15 μL --> 37°C/ 30 min.


Gel Verification

Hover name
Ladder



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2014/06/16&oldid=798040"