Haynes Lab:Notebook/Engineering PC-TFs/2014/06/16

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Summary

Digest All the Things Addendum


Well One: Ladder
Well Two

Reagent Volume Expected:
2520(insert)+3265(V0120 Vector) = 5785bp
Observed:
~7000bp
DNA(BL05 M.P) 8.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 4.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Three

Reagent Volume Expected:
2520(insert)+3265(V0120 Vector) = 5785bp
Observed:
~7000bp
DNA(BL05A) 4.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 8.5 μL
Total 15 μL --> 37°C/ 30 min.

Well Four

Reagent Volume Expected:
1431 (insert) + 3265 (V0120 Vector) = 4696bp
Observed:
~4500
DNA(BL09 M.P.) 8.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 4.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Five

Reagent Volume Expected:
1431 (insert) + 3265 (V0120 Vector) = 4696bp
Observed:
~4000
DNA(BL09B) 4.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 8.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Six

Reagent Volume Expected:
5197bp
Observed:
~4900
DNA(Phosphorylated CMV/MV9 ) 2.0 μL
10X buffer 1.5 μL
dH2O 11.5 μL
Total 15 μL --> 37°C/ 30 min.



Gel Verification

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