Summary
Digest All the Things
Well One: Ladder
Well Two
Reagent
|
Volume
|
Expected: 1. Unknown
|
DNA(CMV/MV9) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
0.0 μL
|
NruI |
0.0 μL
|
dH2O |
8.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Three
Reagent
|
Volume
|
Expected: 1. 5200bp
|
DNA(CMV/MV9) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
NruI |
0.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Four
Reagent
|
Volume
|
Expected: 1. 5200bp
|
DNA(CMV/MV9) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
0.0 μL
|
NruI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Five
Reagent
|
Volume
|
Expected: 1. 710bp, 4490bp
|
DNA(CMV/MV9) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
NruI |
1.0 μL
|
dH2O |
6.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Six
Reagent
|
Volume
|
Expected: 1. BL01 = 2520bp, V0120-2520bp
|
DNA(BL01A) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
SpeI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Seven
Reagent
|
Volume
|
Expected: 1. BL01 = 2520bp, V0120-2520bp
|
DNA(BL01B) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
SpeI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Eight
Reagent
|
Volume
|
Expected: 1. Cameron Lee Gardner 18:00, 10 June 2014 (EDT)
|
DNA(BL05) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
SpeI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Nine
Reagent
|
Volume
|
Expected: 1. Cameron Lee Gardner
|
DNA(BL09) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
SpeI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well 10
Reagent
|
Volume
|
Expected: 1. BL01 = 7720bp
|
DNA(CMV/MV9 & BL01) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
0.0 μL
|
NruI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well 11
Reagent
|
Volume
|
Expected: 1. dependent on ligation direction
|
DNA(CMV/MV9 & BL01) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
NruI |
1.0 μL
|
dH2O |
6.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Gel Verification
|