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Summary Digest All the Things
Well One: Ladder Well Two
Reagent
Volume
Expected:
DNA(CMV/MV9)
5.0 μL
10X buffer
1.5 μL
XbaI
0.0 μL
NruI
0.0 μL
dH2 O
8.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Three
Reagent
Volume
Expected:
DNA(CMV/MV9)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
NruI
0.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Four
Reagent
Volume
Expected:
DNA(CMV/MV9)
5.0 μL
10X buffer
1.5 μL
XbaI
0.0 μL
NruI
1.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Five
Reagent
Volume
Expected:
DNA(CMV/MV9)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
NruI
1.0 μL
dH2 O
6.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Six
Reagent
Volume
Expected:
DNA(BL01A)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
SpeI
1.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Seven
Reagent
Volume
Expected:
DNA(BL01B)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
SpeI
1.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Eight
Reagent
Volume
Expected: Cameron Lee Gardner 18:00, 10 June 2014 (EDT)
DNA(BL05)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
SpeI
1.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well Nine
Reagent
Volume
Expected: Cameron Lee Gardner
DNA(BL09)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
SpeI
1.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well 10
Reagent
Volume
Expected:
DNA(CMV/MV9 & BL01)
5.0 μL
10X buffer
1.5 μL
XbaI
0.0 μL
NruI
1.0 μL
dH2 O
7.5 μL
Total
15 μL --> 37°C/ 30 min.
Well 11
Reagent
Volume
Expected:
DNA(CMV/MV9 & BL01)
5.0 μL
10X buffer
1.5 μL
XbaI
1.0 μL
NruI
1.0 μL
dH2 O
6.5 μL
Total
15 μL --> 37°C/ 30 min.
Gel Verification
