Haynes Lab:Notebook/Engineering PC-TFs/2014/03/21: Difference between revisions

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(Autocreate 2014/03/21 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
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==Summary==
==Summary==
*  
* Evaluating plates and starting liquid culture.
* Plates 2 and 3 (neg control for DH5a-T and experimental for BL21 dried up and were unusable.
* Plate 4 (neg control for BL21 showed zero cell growth.
* Plate 1 (experimental for DH5a) had four colonies.


{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:2014-03-21 11.41.40.jpg|400px|Hover name]];
|- valign="top"
| rowspan="7" | [[Image:2014-03-21 11.41.51.jpg|400px|Hover name]]<br>;
|- valign="top"
| rowspan="7" | [[Image:2014-03-21 11.42.01.jpg|400px|Hover name]];
|- valign="top"
| rowspan="7" | [[Image:2014-03-21 11.42.10.jpg|400px|Hover name]];
|}
* The four colonies were picked and placed into four tubes with 2mL of 100ug/mL amp broth.
* Placed in incubator.


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Revision as of 13:16, 21 March 2014



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Summary

  • Evaluating plates and starting liquid culture.
  • Plates 2 and 3 (neg control for DH5a-T and experimental for BL21 dried up and were unusable.
  • Plate 4 (neg control for BL21 showed zero cell growth.
  • Plate 1 (experimental for DH5a) had four colonies.
Hover name;
Hover name
;
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  • The four colonies were picked and placed into four tubes with 2mL of 100ug/mL amp broth.
  • Placed in incubator.