- Evaluating plates and starting liquid culture.
- Plates 2 and 3 (neg control for DH5a-T and experimental for BL21 dried up and were unusable.
- Plate 4 (neg control for BL21 showed zero cell growth.
- Plate 1 (experimental for DH5a) had four colonies.
- The four colonies were picked and placed into four tubes with 2mL of 100ug/mL amp broth.
- Placed in incubator.