Haynes Lab:Notebook/Engineering PC-TFs/2014/03/21

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Summary

  • Evaluating plates and starting liquid culture.
  • Plates 2 and 3 (neg control for DH5a-T and experimental for BL21 dried up and were unusable.
  • Plate 4 (neg control for BL21 showed zero cell growth.
  • Plate 1 (experimental for DH5a) had four colonies.
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  • The four colonies were picked and placed into four tubes with 2mL of 100ug/mL amp broth.
  • Placed in incubator.


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