Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26

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(Autocreate 2013/09/26 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
(Summary)
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==Summary==
==Summary==
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*  
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*Measure the following into 1.5 mL tubes.
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{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
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| bgcolor=#cfcfcf | '''Reagent'''
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| bgcolor=#cfcfcf | '''15%'''
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| bgcolor=#cfcfcf | '''10%'''
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| bgcolor=#cfcfcf | '''7%'''
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| bgcolor=#cfcfcf | '''5%'''
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| bgcolor=#cfcfcf | '''2%'''
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|-
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| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL
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|-
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| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL
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|-
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| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL
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|-
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| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL
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|}
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Revision as of 21:14, 26 September 2013



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Summary

  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL15 μL15 μL15 μL15 μL
dH2O 35 μL52 μL62 μL68 μL78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL



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