Haynes Lab:Notebook/Engineering PC-TFs/2012/10/26

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(Summary)
(Summary)
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{|border="1" cellpadding="5" cellspacing="0" align="left"
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! scope="col" style="background:#efefef;" | Assembly #
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! scope="col" style="background:#efefef;" | Assembly #
! scope="col" style="background:#efefef;" | Concentration
! scope="col" style="background:#efefef;" | Concentration
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*<u>Gibson Assembly </u>
*<u>Gibson Assembly </u>
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Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.
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{|border="1" cellpadding="5" cellspacing="0" align="left"
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|-
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! scope="col" style="background:#efefef;" |
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! scope="col" style="background:#efefef;" | 1
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! scope="col" style="background:#efefef;" | 2
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|-
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|Gibson Rxn mix
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|15 μL
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|15 μL
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|-
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|DNA fragments
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|5 μL
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|}
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Revision as of 15:26, 30 October 2012

Engineering PC-TFs Main project page
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Summary

Assembly # Concentration
1 24.41 ng/μL
2 17.025 ng/μL
3 42.325 ng/μL
4 16.706 ng/μL
5 45.015 ng/μL











  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL




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