Haynes Lab:Notebook/Engineering PC-TFs/2012/10/26: Difference between revisions
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{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
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! scope="col" style="background:#efefef;" | | ! scope="col" style="background:#efefef;" | Assembly # | ||
! scope="col" style="background:#efefef;" | Concentration | ! scope="col" style="background:#efefef;" | Concentration | ||
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*<u>Gibson Assembly </u> | *<u>Gibson Assembly </u> | ||
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | |||
|- | |||
! scope="col" style="background:#efefef;" | | |||
! scope="col" style="background:#efefef;" | 1 | |||
! scope="col" style="background:#efefef;" | 2 | |||
|- | |||
|Gibson Rxn mix | |||
|15 μL | |||
|15 μL | |||
|- | |||
|DNA fragments | |||
|5 μL | |||
|} | |||
Revision as of 13:26, 30 October 2012
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Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.
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