Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/24: Difference between revisions

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<b>Set up colony PCR for pLux_MRV + LuxR </b>
<b>Set up colony PCR for pLux_MRV + LuxR </b>
:8 completed plasmids and 3 backbones used as a control  
:8 Lux_MRV + LuxR plasmids and 3 Lux_MRV backbones used as a control  


<b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b>  
<b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b>  

Revision as of 16:51, 27 July 2015

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07/24/2015

Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12)

Set up colony PCR for pLux_MRV + LuxR

8 Lux_MRV + LuxR plasmids and 3 Lux_MRV backbones used as a control

Digest verify LasR 1-6, AubR 1-4, Esa 2, 8.

LasR and AubR were verified using KpnI and EcoRI, while EsaR was verified using EcoRI and XbaI
Sent LasR 1, 3, and AubR 4 out for sequencing. It looks like AubR 4 is successful.


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