Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/24: Difference between revisions
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<b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b> | <b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b> | ||
:LasR and AubR were verified using KpnI and EcoRI, while EsaR was verified using EcoRI and XbaI | |||
:[[Image:15.07.24_digest_verifications.TIF.TIF|600px]] | :[[Image:15.07.24_digest_verifications.TIF.TIF|600px]] | ||
:Sent LasR 1, 3, and AubR 4 out for sequencing. It looks like AubR 4 is successful. | :Sent LasR 1, 3, and AubR 4 out for sequencing. It looks like AubR 4 is successful. |
Revision as of 16:36, 27 July 2015
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07/24/2015Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12) Set up colony PCR for pLux_MRV + LuxR
Digest verify LasR 1-6, AubR 1-4, Esa 2, 8.
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