Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/24: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2015/07/24 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(fix raw html notebook nav)
 
(5 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==07//2015==
==07/24/2015==
<font color = purple>
<b>Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12)</b>


<b>Set up colony PCR for pLux_MRV + LuxR </b>
:8 Lux_MRV + LuxR plasmids and 3 Lux_MRV backbones used as a control


<b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b>
:LasR and AubR were verified using KpnI and EcoRI, while EsaR was verified using EcoRI and XbaI
:[[Image:15.07.24_digest_verifications.TIF.TIF|600px]]
:Sent LasR 1, 3, and AubR 4 out for sequencing. It looks like AubR 4 is successful.


</font>
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Latest revision as of 01:03, 27 September 2017

Project name Main project page
Previous entry      Next entry

07/24/2015

Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12)

Set up colony PCR for pLux_MRV + LuxR

8 Lux_MRV + LuxR plasmids and 3 Lux_MRV backbones used as a control

Digest verify LasR 1-6, AubR 1-4, Esa 2, 8.

LasR and AubR were verified using KpnI and EcoRI, while EsaR was verified using EcoRI and XbaI
Sent LasR 1, 3, and AubR 4 out for sequencing. It looks like AubR 4 is successful.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2015/07/24&oldid=1017051"