Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/07
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04/07/2014Trying golden gate for receiver intermediate.
Only digested reactions 1, 2, 4, and 5. Rxn 3 does not have plasmid DNA, the template is a g-block fragment.
Heat inactivated for 5 minutes at 80°C about 15 minutes after taking them out of 37°C, forgot about that....
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