Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/08

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mm/dd/yyyy

Checked second PCR reaction 3 on gel, looks like it's there.
Purify second reaction 3, DpnI digested 20ul reactions 1, 2, 4, 5. Heat inactivated. Cleaned using zymo DNA clean and concentration-5 kit. Added 100ul of DNA binding buffer to DpnI reactions, 125ul DNA binding buffer to 25ul of PCR reaction 3. (don't need to digest Reaction 3 because it wasn't amplifying a vector)



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