Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/06

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==09/06/2013==
==09/06/2013==
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PCR for Golden Gate assemblies<br> Decided to redo everything.
+
PCR for Golden Gate assemblies
<br><br>
<br><br>
{| {{table}}
{| {{table}}
|-
|-
-
|Number||Template||Primer 1||Primer 2||Expected size
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|Number||Template||Primer 1||Primer 2||Expected size||Worked?
|-
|-
-
|1||I13522 73.4||P060||P062||3136
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|1||I13522 73.4||P060||P062||3136||No
|-
|-
-
|2||I13522 73.4||Px||P084||3136
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|2||I13522 73.4||Px||P084||3136||Yes
|-
|-
-
|3||I13522 73.4||Py||Pz||90
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|3||I13522 73.4||Py||Pz||90||Yes
|-
|-
-
|4||I13522 73.4||P060||P062||3136
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|4||I13522 73.4||P060||P062||3136||No
|-
|-
-
|5||I13522 2||Px||P084||3136
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|5||I13522 2||Px||P084||3136||Yes
|-
|-
-
|6||I13522 2||Py||Pz||90
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|6||I13522 2||Py||Pz||90||Yes
|-
|-
-
|7||I13522 73.4||P060||P062||3136
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|7||I13522 73.4||P060||P062||3136||No
|-
|-
-
|8||I13522 3||Px||P084||3136
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|8||I13522 3||Px||P084||3136||Yes
|-
|-
-
|9||I13522 3||Py||Pz||90
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|9||I13522 3||Py||Pz||90||Yes
|-
|-
-
|10||K145254 4||P063||P064||679
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|10||K145254 4||P063||P064||679||Yes
|-
|-
-
|11||K145254 7/24/13||P063||P064||679
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|11||K145254 7/24/13||P063||P064||679||Yes
|-
|-
-
|12||F2620+GFP 7/24/13||P065||P066||817
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|12||F2620+GFP 7/24/13||P065||P066||817||Yes
|-
|-
-
|13||F2620+GFP 7/24/13||P067||P068||56
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|13||F2620+GFP 7/24/13||P067||P068||56||Yes
|-
|-
-
|14||K084007 7/24/13||P069||P070||645
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|14||K084007 7/24/13||P069||P070||645||No
|-
|-
-
|15||K082035 36||P075||P076||703
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|15||K082035 36||P075||P076||703||Yes
|-
|-
-
|16||K092900-E0420 80.5||P077||P078||765
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|16||K092900-E0420 80.5||P077||P078||765||No
|-
|-
-
|17||K092900-E0420 80.5||P079||P080||56
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|17||K092900-E0420 80.5||P079||P080||56||Yes
|-
|-
-
|18||Part B1||P081||P082||980
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|18||Part B1||P081||P082||980||No
|-
|-
-
|19||Part B2||P081||P082||980
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|19||Part B2||P081||P082||980||No
|-
|-
-
|20||Part B3||P081||P082||980
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|20||Part B3||P081||P082||980||No
|-
|-
-
|21||PSB1C3 (1)||P083||P084||3000
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|21||PSB1C3 (1)||P083||P084||3000||Yes
|-
|-
-
|22||PSB1C3 (2)||P083||P084||3000
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|22||PSB1C3 (2)||P083||P084||3000||Yes
|-
|-
-
|23||I13522 2||P060||P062||3136
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|23||I13522 2||P060||P062||3136||No
|-
|-
-
|24||I13522 3||P060||P062||3136
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|24||I13522 3||P060||P062||3136||No
|-
|-
|}
|}
<br>
<br>
 +
<br>
 +
 +
[[image:13.09.07_GG_PCR_top_edited.png‎ |600px|GG PCR]]
 +
<br><br>
 +
[[image:13.09.07_GG_PCR_bottom.png‎ |650px|GG PCR]]
 +
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Revision as of 15:16, 12 September 2013

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09/06/2013

PCR for Golden Gate assemblies

NumberTemplatePrimer 1Primer 2Expected sizeWorked?
1I13522 73.4P060P0623136No
2I13522 73.4PxP0843136Yes
3I13522 73.4PyPz90Yes
4I13522 73.4P060P0623136No
5I13522 2PxP0843136Yes
6I13522 2PyPz90Yes
7I13522 73.4P060P0623136No
8I13522 3PxP0843136Yes
9I13522 3PyPz90Yes
10K145254 4P063P064679Yes
11K145254 7/24/13P063P064679Yes
12F2620+GFP 7/24/13P065P066817Yes
13F2620+GFP 7/24/13P067P06856Yes
14K084007 7/24/13P069P070645No
15K082035 36P075P076703Yes
16K092900-E0420 80.5P077P078765No
17K092900-E0420 80.5P079P08056Yes
18Part B1P081P082980No
19Part B2P081P082980No
20Part B3P081P082980No
21PSB1C3 (1)P083P0843000Yes
22PSB1C3 (2)P083P0843000Yes
23I13522 2P060P0623136No
24I13522 3P060P0623136No



GG PCR

GG PCR



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