09/06/2013
PCR for Golden Gate assemblies
Number |
Template |
Primer 1 |
Primer 2 |
Expected size |
Worked?
|
1 |
I13522 73.4 |
P060 |
P062 |
3136 |
No
|
2 |
I13522 73.4 |
P095 |
P084 |
3136 |
Yes
|
3 |
I13522 73.4 |
P093 |
P094 |
90 |
Yes
|
4 |
I13522 73.4 |
P060 |
P062 |
3136 |
No
|
5 |
I13522 2 |
P095 |
P084 |
3136 |
Yes
|
6 |
I13522 2 |
P093 |
P094 |
90 |
Yes
|
7 |
I13522 73.4 |
P060 |
P062 |
3136 |
No
|
8 |
I13522 3 |
P095 |
P084 |
3136 |
Yes
|
9 |
I13522 3 |
P093 |
P094 |
90 |
Yes
|
10 |
K145254 4 |
P063 |
P064 |
679 |
Yes
|
11 |
K145254 7/24/13 |
P063 |
P064 |
679 |
Yes
|
12 |
F2620+GFP 7/24/13 |
P065 |
P066 |
817 |
Yes
|
13 |
F2620+GFP 7/24/13 |
P067 |
P068 |
56 |
Yes
|
14 |
K084007 7/24/13 |
P069 |
P070 |
645 |
No
|
15 |
K082035 36 |
P075 |
P076 |
703 |
Yes
|
16 |
K092900-E0420 80.5 |
P077 |
P078 |
765 |
No
|
17 |
K092900-E0420 80.5 |
P079 |
P080 |
56 |
Yes
|
18 |
Part B1 |
P081 |
P082 |
980 |
No
|
19 |
Part B2 |
P081 |
P082 |
980 |
No
|
20 |
Part B3 |
P081 |
P082 |
980 |
No
|
21 |
PSB1C3 (1) |
P083 |
P084 |
3000 |
Yes
|
22 |
PSB1C3 (2) |
P083 |
P084 |
3000 |
Yes
|
23 |
I13522 2 |
P060 |
P062 |
3136 |
No
|
24 |
I13522 3 |
P060 |
P062 |
3136 |
No
|
|