Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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==Entry title== | ==Entry title== | ||
minipreps | minipreps | ||
streak | streak | ||
cut and extract and clone | cut and extract and clone | ||
pull out promoters + RBS from registry | pull out promoters + RBS from registry | ||
gel extract and ligate and transform part 6 | gel extract and ligate and transform part 6 | ||
Line 25: | Line 29: | ||
transformed RBS+LuxI and RBS+LasI | transformed RBS+LuxI and RBS+LasI | ||
{| {{table}} | |||
|- | |||
|Part||260||280||260/280||ng/ul | |||
|- | |||
|K079021||0.131||0.07||1.836||130.892 | |||
|- | |||
|I13522||0.125||0.068||1.836||125.239 | |||
|- | |||
|K079020||0.086||0.046||1.855||85.913 | |||
|- | |||
|J45200||0.081||0.045||1.81||80.868 | |||
|- | |||
|J04450||0.071||0.038||1.859||70.782 | |||
|- | |||
|} | |||
Revision as of 13:35, 28 May 2013
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Entry titleminipreps streak cut and extract and clone pull out promoters + RBS from registry gel extract and ligate and transform part 6
cut K081016 and R0071 from above, ran on a gel, didn't work Ernesto ran colony PCR on a bunch of mini preps and colonies transformed RBS+LuxI and RBS+LasI
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