Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07

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minipreps

streak

cut and extract and clone

pull out promoters + RBS from registry

gel extract and ligate and transform part 6


miniprep C0061 1 and 2 C0062 K081016 that haynes grew up, streaked on plate (she found by PCR) R0071 that haynes grew up, streaking on plate " "

cut K081016 and R0071 from above, ran on a gel, didn't work

Ernesto ran colony PCR on a bunch of mini preps and colonies

transformed RBS+LuxI and RBS+LasI

Part260280260/280ng/ul
K0790210.1310.071.836130.892
I135220.1250.0681.836125.239
K0790200.0860.0461.85585.913
J452000.0810.0451.8180.868
J044500.0710.0381.85970.782



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