Haynes Lab:Notebook/CRISPR Editing/2015/10/03: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==03//2015==
==10/03/2015==
 
PCR of siRNA samples wiht P197/198
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''siRNA'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 200ng'''
| align="center" style="background:#f0f0f0;"|'''5ul water-200ng'''
|-
| Gal4-EED||dox/puro||EZH2||g025||1||179.613||1.11||3.89
|-
| Gal4-EED||dox/puro||EZH2||g025||2||173.433||1.15||3.85
|-
| Gal4-EED||dox/puro||EZH2||g025||3||104.127||1.92||3.08
|-
| Gal4-EED||dox/puro||Blank||g025||1||201.109||0.99||4.01
|-
| Gal4-EED||dox/puro||Blank||g025||2||233.53||0.86||4.14
|-
| Gal4-EED||dox/puro||Blank||g025||3||174.093||1.15||3.85
|-
| Gal4-EED||dox/puro||EZH2||g034||1||96.926||2.06||2.94
|-
| Gal4-EED||dox/puro||EZH2||g034||2||62.154||3.22||1.78
|-
| Gal4-EED||dox/puro||EZH2||g034||3||123.599||1.62||3.38
|-
| Gal4-EED||dox/puro||Blank||g034||1||214.213||0.93||4.07
|-
| Gal4-EED||dox/puro||Blank||g034||2||213.397||0.94||4.06
|-
| Gal4-EED||dox/puro||Blank||g034||3||182.355||1.10||3.90
|}




Latest revision as of 01:13, 27 September 2017

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10/03/2015

PCR of siRNA samples wiht P197/198

Cell type drug siRNA gRNA rep ng/µL ul for 200ng 5ul water-200ng
Gal4-EED dox/puro EZH2 g025 1 179.613 1.11 3.89
Gal4-EED dox/puro EZH2 g025 2 173.433 1.15 3.85
Gal4-EED dox/puro EZH2 g025 3 104.127 1.92 3.08
Gal4-EED dox/puro Blank g025 1 201.109 0.99 4.01
Gal4-EED dox/puro Blank g025 2 233.53 0.86 4.14
Gal4-EED dox/puro Blank g025 3 174.093 1.15 3.85
Gal4-EED dox/puro EZH2 g034 1 96.926 2.06 2.94
Gal4-EED dox/puro EZH2 g034 2 62.154 3.22 1.78
Gal4-EED dox/puro EZH2 g034 3 123.599 1.62 3.38
Gal4-EED dox/puro Blank g034 1 214.213 0.93 4.07
Gal4-EED dox/puro Blank g034 2 213.397 0.94 4.06
Gal4-EED dox/puro Blank g034 3 182.355 1.10 3.90



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