Haynes Lab:Notebook/CRISPR Editing/2015/10/06

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10/06/2015

Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel

no bands in neg ctrl, all other lanes look great



Col pur Gal4 puro/dox g054 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EEDpurog05410.10.0541.87100.1893.991.509.51
Gal4-EEDpurog05420.090.0471.9190.4664.421.509.08
Gal4-EEDpurog05430.1050.0571.862105.53.791.509.71
Gal4-EEDdoxg05410.1040.0541.919103.7273.861.509.64
Gal4-EEDdoxg05420.1150.0631.825114.9853.481.5010.02
Gal4-EEDdoxg05430.0920.0491.89192.0384.351.509.15



Col pur Gal4 puro/dox g055 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EEDpurog05510.0680.0351.9368.2295.861.507.64
Gal4-EEDpurog05520.0720.0381.8771.6185.591.507.91
Gal4-EEDpurog05530.0790.0421.88278.8265.071.508.43
Gal4-EEDdoxg05510.070.0381.83969.7415.741.507.76
Gal4-EEDdoxg05520.0740.0391.87573.7145.431.508.07
Gal4-EEDdoxg05530.0740.0421.77374.4425.371.508.13


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