Haynes Lab:Notebook/CRISPR Editing/2015/09/11: Difference between revisions

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(Autocreate 2015/09/11 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==09/11/2015==
 
Ran failed nested PCR of g031 on gel next to neg ctrl and all P197 198 g025 and g044 PCRs. No band in neg ctrl for P197 198 and all other lanes looked great. <br>
PCR purified g031 nested PCRs, set up annealing rxn for surveyor.


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||puro||g031||1||0.052||0.03||1.733||51.652||7.74||1.50||5.76
|-
| Gal4-EED||puro||g031||2||0.078||0.043||1.795||77.923||5.13||1.50||8.37
|-
| Gal4-EED||puro||g031||3||0.066||0.037||1.815||66.314||6.03||1.50||7.47
|-
| Gal4-EED||dox||g031||1||0.073||0.041||1.777||72.898||5.49||1.50||8.01
|-
| Gal4-EED||dox||g031||2||0.092||0.049||1.859||91.767||4.36||1.50||9.14
|-
| Gal4-EED||dox||g031||3||0.073||0.041||1.773||73.146||5.47||1.50||8.03
|}


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Revision as of 09:33, 11 September 2015

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09/11/2015

Ran failed nested PCR of g031 on gel next to neg ctrl and all P197 198 g025 and g044 PCRs. No band in neg ctrl for P197 198 and all other lanes looked great.
PCR purified g031 nested PCRs, set up annealing rxn for surveyor.

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4-EED puro g031 1 0.052 0.03 1.733 51.652 7.74 1.50 5.76
Gal4-EED puro g031 2 0.078 0.043 1.795 77.923 5.13 1.50 8.37
Gal4-EED puro g031 3 0.066 0.037 1.815 66.314 6.03 1.50 7.47
Gal4-EED dox g031 1 0.073 0.041 1.777 72.898 5.49 1.50 8.01
Gal4-EED dox g031 2 0.092 0.049 1.859 91.767 4.36 1.50 9.14
Gal4-EED dox g031 3 0.073 0.041 1.773 73.146 5.47 1.50 8.03


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