Haynes Lab:Notebook/CRISPR Editing/2015/09/11

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09/11/2015

Ran failed nested PCR of g031 on gel next to neg ctrl and all P197 198 g025 and g044 PCRs. No band in neg ctrl for P197 198 and all other lanes looked great.
PCR purified g031 nested PCRs, set up annealing rxn for surveyor.

Cell type drug gRNA rep 260 280 260/280 ng/┬ÁL ul for 400ng ul ann buff ul water
Gal4-EEDpurog03110.0520.031.73351.6527.741.505.76
Gal4-EEDpurog03120.0780.0431.79577.9235.131.508.37
Gal4-EEDpurog03130.0660.0371.81566.3146.031.507.47
Gal4-EEDdoxg03110.0730.0411.77772.8985.491.508.01
Gal4-EEDdoxg03120.0920.0491.85991.7674.361.509.14
Gal4-EEDdoxg03130.0730.0411.77373.1465.471.508.03


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