Haynes Lab:Notebook/CRISPR Editing/2015/03/06

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03/06/2015

miniprep of pMax GFP 6.2 from Lonza and KAH60

plasmid colony 260 280 260/280 ng/µL ul for 05.ng
pMax GFP 6.2 1 0.552 0.299 1.848 551.779 0.906159894
pMax GFP 6.2 2 0.348 0.187 1.861 347.553 1.438629504
KAH60 1 0.102 0.056 1.817 101.806 4.911301888
KAH60 2 0.278 0.148 1.878 278.411 1.795906053


plasmid ng/µL ul for 05.ng g044 403.212 1.240042459 g031 288.187 1.734984576

plated cells look great, all wells are about 90% confluent. Will proceed with all 48 transfections

KAH60 transfections


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