Haynes Lab:Notebook/CRISPR Editing/2015/01/25: Difference between revisions
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Picked KAH79 and KAH126<br><br> | Picked KAH79 and KAH126<br><br> | ||
'''Transfected each well of two 12-well plates with 1ug of plasmid DNA.''' | '''Transfected each well of two 12-well plates with 1ug of plasmid DNA.''' | ||
The cells looked way better than last time. Luc14s were about 80-90% confluent while the gal4-eeds were about 90-95% confluent. The media for the gal4s was starting to turn orange so I added 1mL of media to each well (added dox to the gal4s) | |||
'''Step 0: Prep DNA'''<br> | '''Step 0: Prep DNA'''<br> | ||
definitely g023, g034 | definitely g023, g034 | ||
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:Incubate for 30 minutes at RT | :Incubate for 30 minutes at RT | ||
'''Steo 4: Add complexes dropwise to each well''' | '''Steo 4: Add complexes dropwise to each well''' | ||
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Everything went well, finished about 4:30. | |||
Revision as of 16:33, 25 January 2015
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01/25/2015Picked KAH79 and KAH126
MM1: Mix PLUS reagent with optimem
MM 2: Lipo LTX and optimem
Step 3: Mix in Lipo LTX
Steo 4: Add complexes dropwise to each well
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