Haynes Lab:Notebook/CRISPR Editing/2015/01/24

Project name

Main project page Previous entry      Next entry

01/24/2015 Cell line gRNA Rep 260 280 260/280 ng/µL Luc14 g045 1 0.182 0.09 2.013 181.634 Luc14 g045 2 0.246 0.122 2.012 246.109 Luc14 g045 3 0.175 0.089 1.953 174.603 GAL4-EED +dox g045 1 0.074 0.036 2.058 74.461 GAL4-EED +dox g045 2 0.083 0.04 2.071 82.614 GAL4-EED +dox g045 3 0.059 0.03 1.969 58.964 Luc14 g046 1 0.255 0.125 2.036 255.228 Luc14 g046 2 0.171 0.085 2.01 170.592 Luc14 g046 3 0.144 0.072 2.003 144.251 GAL4-EED +dox g046 1 0.073 0.038 1.927 72.999 GAL4-EED +dox g046 2 0.079 0.04 1.954 78.598 GAL4-EED +dox g046 3 0.077 0.04 1.926 77.323 Plated for transfections Used 12-well plates, have enough wells to do 3 gRNAs. Plated GAL4-EED+dox cells at 80% confluency.