Haynes Lab:Notebook/CRISPR Editing/2014/09/10: Difference between revisions
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==09/10/2014== | ==09/10/2014== | ||
Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow.<br><br> | Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow.<br><br> | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''PCR mix''' | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Band''' | |||
|- | |||
| 1||Phusion||P196||P198||1522 | |||
|- | |||
| 2||Phusion||P197||P198||1660 | |||
|- | |||
| 3||Taq 2x||P196||P198||1522 | |||
|- | |||
| 4||Taq 2x||P197||P198||1660 | |||
|} | |||
<br><br> | |||
Expected band sizes: | Expected band sizes: | ||
:1522 for lanes 1 & 3 | :1522 for lanes 1 & 3 |
Revision as of 08:43, 15 September 2014
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09/10/2014Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow.
Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template.
Annealing Temp 57, et 1min
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