Haynes Lab:Notebook/CRISPR Editing/2014/09/08

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09/08/2014

Extracted genomic DNA from CRISPR on Chromatin samples using QiaAmp kit. All concentrations look great. Samples gRNA 27 +dox rep 1 and gRNA 27 -dox rep 1 have 260/280 over 2. Did extractions in batches: first batch was 1-12, second was 13-20, and third was 21-25.

Sample Read# 260 280 260/280 ng/┬ÁL
gRNA 20 +dox rep 10.3170.1621.959317.235
gRNA 20 +dox rep 20.2570.131.971256.97
gRNA 20 -dox rep 10.2520.1291.957252.053
gRNA 20 -dox rep 20.1860.0981.896186.423
gRNA 21 +dox rep 10.1890.0961.961189.104
gRNA 21 +dox rep 20.3160.1621.95315.556
gRNA 21 -dox rep 10.240.1241.939240.117
gRNA 21 -dox rep 20.2280.1211.891228.478
gRNA 22 +dox rep 10.1890.0971.947189.43
gRNA 22 +dox rep 20.3270.1651.978326.537
gRNA 22 -dox rep 10.290.151.939290.088
gRNA 22 -dox rep 20.2930.1571.869293.275
gRNA 27 +dox rep 10.3910.1952.004391.309
gRNA 27 +dox rep 20.2780.1421.952277.931
gRNA 27 -dox rep 10.2350.1172.006235.313
gRNA 27 -dox rep 20.2060.1051.964206.214
gRNA 29 +dox rep 10.330.1651.994329.933
gRNA 29 +dox rep 20.2750.141.97275.026
gRNA 29 -dox rep 10.2120.1091.953211.985
gRNA 29 -dox rep 20.20.1031.942200.274
Luc14 200.4220.2131.977421.658
Luc14 210.3680.1911.932368.465
Luc14 220.2740.141.966274.319
Luc14 270.2210.1151.924221.046
Luc14 290.1560.0831.888156.078



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