09/08/2014
Extracted genomic DNA from CRISPR on Chromatin samples using QiaAmp kit. All concentrations look great. Samples gRNA 27 +dox rep 1 and gRNA 27 -dox rep 1 have 260/280 over 2. Did extractions in batches: first batch was 1-12, second was 13-20, and third was 21-25.
| Sample Read#
|
260
|
280
|
260/280
|
ng/µL
|
| gRNA 20 +dox rep 1 |
0.317 |
0.162 |
1.959 |
317.235
|
| gRNA 20 +dox rep 2 |
0.257 |
0.13 |
1.971 |
256.97
|
| gRNA 20 -dox rep 1 |
0.252 |
0.129 |
1.957 |
252.053
|
| gRNA 20 -dox rep 2 |
0.186 |
0.098 |
1.896 |
186.423
|
| gRNA 21 +dox rep 1 |
0.189 |
0.096 |
1.961 |
189.104
|
| gRNA 21 +dox rep 2 |
0.316 |
0.162 |
1.95 |
315.556
|
| gRNA 21 -dox rep 1 |
0.24 |
0.124 |
1.939 |
240.117
|
| gRNA 21 -dox rep 2 |
0.228 |
0.121 |
1.891 |
228.478
|
| gRNA 22 +dox rep 1 |
0.189 |
0.097 |
1.947 |
189.43
|
| gRNA 22 +dox rep 2 |
0.327 |
0.165 |
1.978 |
326.537
|
| gRNA 22 -dox rep 1 |
0.29 |
0.15 |
1.939 |
290.088
|
| gRNA 22 -dox rep 2 |
0.293 |
0.157 |
1.869 |
293.275
|
| gRNA 27 +dox rep 1 |
0.391 |
0.195 |
2.004 |
391.309
|
| gRNA 27 +dox rep 2 |
0.278 |
0.142 |
1.952 |
277.931
|
| gRNA 27 -dox rep 1 |
0.235 |
0.117 |
2.006 |
235.313
|
| gRNA 27 -dox rep 2 |
0.206 |
0.105 |
1.964 |
206.214
|
| gRNA 29 +dox rep 1 |
0.33 |
0.165 |
1.994 |
329.933
|
| gRNA 29 +dox rep 2 |
0.275 |
0.14 |
1.97 |
275.026
|
| gRNA 29 -dox rep 1 |
0.212 |
0.109 |
1.953 |
211.985
|
| gRNA 29 -dox rep 2 |
0.2 |
0.103 |
1.942 |
200.274
|
| Luc14 20 |
0.422 |
0.213 |
1.977 |
421.658
|
| Luc14 21 |
0.368 |
0.191 |
1.932 |
368.465
|
| Luc14 22 |
0.274 |
0.14 |
1.966 |
274.319
|
| Luc14 27 |
0.221 |
0.115 |
1.924 |
221.046
|
| Luc14 29 |
0.156 |
0.083 |
1.888 |
156.078
|
|
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