Haynes Lab:Notebook/CRISPR Editing/2014/09/10: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/09/10 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==09/10/2014== | ||
Checked Luc->AmCyan PCR donor I transfected with on a gel. The correct band is there but there's other bands too so I will do the PCR again and raise the at and reduced the amount of template to try to get rid of other bands.<br> | |||
Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Exp band''' | |||
|- | |||
| 4c 3||4c 2||P192||P195||918 | |||
|} | |||
Annealing Temp 57, et 1min | |||
<br> | |||
Revision as of 16:33, 10 September 2014
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09/10/2014Checked Luc->AmCyan PCR donor I transfected with on a gel. The correct band is there but there's other bands too so I will do the PCR again and raise the at and reduced the amount of template to try to get rid of other bands. Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template.
Annealing Temp 57, et 1min
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