Haynes:Protocols: Difference between revisions
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=Recipes= | =Recipes= | ||
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''' | '''Bromo-Blue/X-cyanol Loading Buffer, 10X''' {{hide| | ||
Formula: [30% glycerol; 12.5 mg/ml bromophenol blue; 12.5 mg/ml xylene cyanol] | |||
Volume: 20 mL | |||
* Caesin tryptone, 10 g | * Caesin tryptone, 10 g | ||
* Yeast extract, 5 g | * Yeast extract, 5 g | ||
* NaCl, 5 g | * NaCl, 5 g | ||
* 1 N NaOH, 1 mL | * 1 N NaOH, 1 mL | ||
Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl. | |||
}} | }} | ||
'''LB Broth (Lennox)''' {{hide| | '''LB Broth (Lennox)''' {{hide| | ||
Volume: 1 L | |||
* Caesin tryptone, 10 g | * Caesin tryptone, 10 g | ||
* Yeast extract, 5 g | * Yeast extract, 5 g | ||
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}} | }} | ||
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Revision as of 11:45, 19 January 2012
Protocols
BioBrick Assembly
Cell Culture: Bacteria
Cell Culture: Mammalian
- Coming soon
Resources at OpenWetWare
- Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.
Recipes
Bromo-Blue/X-cyanol Loading Buffer, 10X
Formula: [30% glycerol; 12.5 mg/ml bromophenol blue; 12.5 mg/ml xylene cyanol] Volume: 20 mL
- Caesin tryptone, 10 g
- Yeast extract, 5 g
- NaCl, 5 g
- 1 N NaOH, 1 mL
Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
LB Broth (Lennox)
Volume: 1 L
- Caesin tryptone, 10 g
- Yeast extract, 5 g
- NaCl, 5 g
- 1 N NaOH, 1 mL
Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.
