Glutaraldehyde (Pentanedial)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(properties)
Line 1: Line 1:
[[Image:Glutaraldehyde chemical structure.png|thumb|right|Chemical structure of glutaraldehyde (1,5-pentane-dial)]]
[[Image:Glutaraldehyde chemical structure.png|thumb|right|Chemical structure of glutaraldehyde (1,5-pentane-dial)]]
-
'''Glutaraldehyde''' (1,5-pentanedial) is a common fixative in biology. It is used to reduce degradation in cells, tissues, and entire organisms before further experiments like electron microscopy.
+
'''Glutaraldehyde''' (1,5-pentanedial, abbreviated as '''GA''') is a common fixative in biology. It is used to reduce degradation in cells, tissues, and entire organisms before further experiments like electron microscopy. Fixation occurs by crosslinking (creating covalent chemical bonds between proteins in/on cells). GA is similar to another common cross-linking fixative, [[PFA]].  
== Properties ==
== Properties ==
-
 
+
* glutaraldehyde is a potent, cross-linking fixative
-
* Glutaraldehyde is a potent fixative, and should only be used in a chemical fume hood. Do not dispose of excess fixative or glutaraldehyde solution in the sink.
+
* bridges larger distances than PFA
-
* Glutaraldehyde causes more autofluorescence than [[PFA]]. See [[Griffin:Immunofluorescence_Cell_Staining#Fixative-induced_Fluorescence|Griffin IHC notes]].
+
* crosslinks are irreversible unlike those of PFA
 +
* larger molecule than methanal from PFA & therefore slower penetration into tissue
 +
* '''toxic & irritating''' - handle in a chemical fume hood; do not dispose of in the sink
 +
* causes more autofluorescence than PFA; see [[Griffin:Immunofluorescence_Cell_Staining#Fixative-induced_Fluorescence|Griffin IHC notes]]
== Uses ==
== Uses ==
-
 
* Glutaraldehyde is used to fix specimen before [[electron microscopy]] where it is employed alone or mixed with [[polymethanal (paraformaldehyde)]] as the first of 2 fixations followed by osmium tetroxide.
* Glutaraldehyde is used to fix specimen before [[electron microscopy]] where it is employed alone or mixed with [[polymethanal (paraformaldehyde)]] as the first of 2 fixations followed by osmium tetroxide.
* Glutaraldehyde is used as an amine cross-linker.
* Glutaraldehyde is used as an amine cross-linker.
Line 22: Line 24:
* [[DIYbio/FAQ/Methods|Glutaraldehyde as disinfectant]]
* [[DIYbio/FAQ/Methods|Glutaraldehyde as disinfectant]]
-
* [http://en.wikipedia.org/wiki/Glutaraldehyde Glutaraldehyde Wikipedia entry]
+
* [http://en.wikipedia.org/wiki/Glutaraldehyde Glutaraldehyde] and [http://en.wikipedia.org/wiki/Fixation_%28histology%29 Fixation] Wikipedia entries

Revision as of 08:52, 3 November 2011

Chemical structure of glutaraldehyde (1,5-pentane-dial)
Chemical structure of glutaraldehyde (1,5-pentane-dial)

Glutaraldehyde (1,5-pentanedial, abbreviated as GA) is a common fixative in biology. It is used to reduce degradation in cells, tissues, and entire organisms before further experiments like electron microscopy. Fixation occurs by crosslinking (creating covalent chemical bonds between proteins in/on cells). GA is similar to another common cross-linking fixative, PFA.

Contents

Properties

  • glutaraldehyde is a potent, cross-linking fixative
  • bridges larger distances than PFA
  • crosslinks are irreversible unlike those of PFA
  • larger molecule than methanal from PFA & therefore slower penetration into tissue
  • toxic & irritating - handle in a chemical fume hood; do not dispose of in the sink
  • causes more autofluorescence than PFA; see Griffin IHC notes

Uses

  • Glutaraldehyde is used to fix specimen before electron microscopy where it is employed alone or mixed with polymethanal (paraformaldehyde) as the first of 2 fixations followed by osmium tetroxide.
  • Glutaraldehyde is used as an amine cross-linker.
  • Glutaraldehyde is used in SDS-PAGE to fix/crosslink proteins and peptides prior to staining. Gels are treated with a 5% solution for ~30 min, after which it must be thoroughly washed to remove the yellow stain brought about by reacting with free Tris. Alternatively, gels can be washed before fixation.

Recipes

Links

Personal tools