Gill:Protocols

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===Microbiology and Molecular Biology===
===Microbiology and Molecular Biology===
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[[Gill:Chung cells |Chung cells]] <br>
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[[Gill:Confirming phage via gel electrophoresis |Confirming phage via gel electrophoresis]] <br>
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[[Gill:Chung cells |Chung cells]]
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[[Gill:Making frozen permanents |Making frozen permanents]] <br>
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[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] <br>
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[[Gill:Confirming phage via gel electrophoresis |Confirming phage via gel electrophoresis]]
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 +
[[Gill:Making frozen permanents |Making frozen permanents]]
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[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]]
===Solutions and Media Preparation===
===Solutions and Media Preparation===

Revision as of 17:40, 7 November 2013

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Protocols

Microbiology and Molecular Biology

Chung cells

Confirming phage via gel electrophoresis

Making frozen permanents

S. aureus genomic DNA isolation

Solutions and Media Preparation

Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

LB agar

LB broth

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

10 X M9 salts

10 X M9 salts + glucose

S liquid medium

TNA (Tryptone Nutrient Agar)

TNA-SA

TNB (Tryptone Nutrient Broth)

Tris-HCl

TSA (Tryptone Soy Agar)

TSB (Tryptone Soy Broth)

T-top (0.5% agar)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts

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