Gill:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 11: | Line 11: | ||
===Solutions and Media Preparation=== | ===Solutions and Media Preparation=== | ||
[[Gill: | [[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]] | ||
[[Gill: | [[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]] | ||
[[Gill: | [[Gill:LB agar |LB agar]] | ||
[[Gill: | [[Gill:LB broth |LB broth]] | ||
[[Gill: | [[Gill:M9 agar |M9 agar]] | ||
[[Gill: | [[Gill:Supplemented M9 agar (M9sup) |Supplemented M9 agar (M9sup)]] | ||
[[Gill: | [[Gill:Supplemented M9 broth (M9sup) |Supplemented M9 broth (M9sup)]] | ||
[[Gill: | [[Gill:10 X M9 salts |10 X M9 salts]] | ||
[[Gill: | [[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]] | ||
[[Gill: | [[Gill:S liquid medium |S liquid medium]] | ||
[[Gill: | [[Gill:TNA (Tryptone Nutrient Agar) |TNA (Tryptone Nutrient Agar)]] | ||
[[Gill: | [[Gill:TNA-SA |TNA-SA]] | ||
[[Gill: | [[Gill:TNB (Tryptone Nutrient Broth) |TNB (Tryptone Nutrient Broth)]] | ||
[[Gill: | [[Gill:Tris-HCl |Tris-HCl]] | ||
[[Gill: | [[Gill:TSA (Tryptone Soy Agar) |TSA (Tryptone Soy Agar)]] | ||
[[Gill: | [[Gill:TSB (Tryptone Soy Broth) |TSB (Tryptone Soy Broth)]] | ||
[[Gill: | [[Gill:T-top (0.5% agar) |T-top (0.5% agar)]] | ||
[[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]] | [[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]] | ||
[[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]] | [[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]] |
Revision as of 14:20, 7 November 2013
Protocols
Microbiology
Chung cells
Confirming phage via gel electrophoresis
Making frozen permanents
S. aureus genomic DNA isolation
Solutions and Media Preparation
10x Lambda diluent (no gelatin)