Etchevers:Notebook/Genomics of hNCC/2008/10/22

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Petit bout de papier

Looked at rabbit cornea for the male 980; it may have been the control rabbit; the R and L corneas looked identical. Some auto-fluorescence in collagen layers (PAF fixed, no anti-aldehyde treatment), scar not obvious to find in either case either. May need to cut more eyes, may need to deparaffinate - but I don't think so.

Took photos of different cultures after fixation on light microscope.

Panserin 401 medium. Cephalic hNCC at 20x.

Panserin 401 medium. Cephalic hNCC at 20x.

CHO C6000 medium. Cephalic hNCC at 20x.

hEScGro medium. Cephalic hNCC at 20x.

hEScGro medium. Truncal hNCC at 20x.

hEScGro medium. Truncal hNCC at 20x, another shot.

hEScGro medium. Truncal hNCC at 20x, another shot.

Stempan medium. Cephalic hNCC at 20x.

Stempan medium again, Cephalic hNCC at 20x, arrows showing spaces cells seem to avoid on plastic.

Stempan medium again, Cephalic hNCC at 20x, arrow showing an apparent neurite or other ramification.

Stempan medium then 3 days of neural differentiation medium from Sigma. Cephalic hNCC at 20x.

Stempan medium then 3 days of neural differentiation medium from Sigma. Cephalic hNCC at 20x.

  • Heather 05:55, 7 November 2008 (EST):