Endy:Screening plasmid/Notebook: Difference between revisions
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(New page: ==Characterization of Empty Screening Plasmid 1.0== *Endy:Screening plasmid/v1.0/Hairpin - characterize effectiveness of the hairpin to stabilize the mRFP1 transcript *[[Endy:Screening...) |
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*[[Endy:Screening plasmid/v1.0/Characterization protocol]] | *[[Endy:Screening plasmid/v1.0/Characterization protocol]] | ||
To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output). | To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output). | ||
==Screening Plasmid 1.5== | |||
*Andrzej's constructs of F2620/1 + GFP were wrong due to the primer being designed incorrectly (it was a BB tail that bound to GFP without including the RBS). | |||
*F2620/1 + GFP/RFP need to be sequenced but the preps are available. | |||
Revision as of 08:46, 12 June 2007
Characterization of Empty Screening Plasmid 1.0
- Endy:Screening plasmid/v1.0/Hairpin - characterize effectiveness of the hairpin to stabilize the mRFP1 transcript
- Endy:Screening plasmid/v1.0/RNAseE sites - characterize effectiveness of the RNAseE sites
- Endy:Screening plasmid/v1.0/Growth rates - characterization of growth rates under different induction levels / conditions
- Endy:Screening plasmid/v1.0/Steady state - evaluate expression levels at various time points following induction.
- Endy:Screening plasmid/v1.0/Characterization protocol
To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output).
Screening Plasmid 1.5
- Andrzej's constructs of F2620/1 + GFP were wrong due to the primer being designed incorrectly (it was a BB tail that bound to GFP without including the RBS).
- F2620/1 + GFP/RFP need to be sequenced but the preps are available.
