Endy:Screening plasmid/v1.0/Hairpin

From OpenWetWare

Results

Constructs

We are building constructs with and without the hairpin and with and without the terminator. These are downstream of two different strength constitutive promoters (<bbpart>R0040</bbpart> and <bbpart>R0050</bbpart>).

• <bbpart>I13521</bbpart> - R0040.B0034.E1010.B0015
• <bbpart>I13572</bbpart> - R0040.hairpin.B0034.E1010
• <bbpart>I13573</bbpart> - R0040.hairpin.B0034.E1010.B0015
• <bbpart>I13574</bbpart> - R0050.B0034.E1010.B0015
• <bbpart>I13575</bbpart> - R0050.hairpin.B0034.E1010.B0015
• <bbpart>I13576</bbpart> - R0050.hairpin.B0034.E1010

Related parts

These haven't been cloned into BB, but were just PCR'd out

• <bbpart>I13570</bbpart> - hairpin.B0034.E1010
• <bbpart>I13571</bbpart> - hairpin.B0034.E1010.B0015
• <bbpart>I13577</bbpart> - hairpin (this hasn't been PCR'd)

Assembly status

Sequence Verified

• <bbpart>I13521</bbpart> - R0040.B0034.E1010.B0015
• <bbpart>I13573</bbpart> - R0040.hairpin.B0034.E1010.B0015
• <bbpart>I13575</bbpart> - R0050.hairpin.B0034.E1010.B0015
• <bbpart>I13574</bbpart> - R0050.B0034.E1010.B0015
• We also have a version of <bbpart>I13572</bbpart> with AC inserted in position 3 of the hairpin (otherwise perfect).

Failed

• <bbpart>I13572</bbpart> - R0040.hairpin.B0034.E1010
  • AC insert in position 3 of the hairpin
• <bbpart>I13576</bbpart> - R0050.hairpin.B0034.E1010
  • Failed at ligation/transformation step (no colonies).