Endy:Screening plasmid/v1.0/Hairpin

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Constructs

We are building constructs with and without the hairpin and with and without the terminator. These are downstream of two different strength constitutive promoters (<bbpart>R0040</bbpart> and <bbpart>R0050</bbpart>).

  • <bbpart>I13521</bbpart> - R0040.B0034.E1010.B0015
  • <bbpart>I13572</bbpart> - R0040.hairpin.B0034.E1010
  • <bbpart>I13573</bbpart> - R0040.hairpin.B0034.E1010.B0015
  • <bbpart>I13574</bbpart> - R0050.B0034.E1010.B0015
  • <bbpart>I13575</bbpart> - R0050.hairpin.B0034.E1010.B0015
  • <bbpart>I13576</bbpart> - R0050.hairpin.B0034.E1010

Related parts

These haven't been cloned into BB, but were just PCR'd out

  • <bbpart>I13570</bbpart> - hairpin.B0034.E1010
  • <bbpart>I13571</bbpart> - hairpin.B0034.E1010.B0015
  • <bbpart>I13577</bbpart> - hairpin (this hasn't been PCR'd)

Assembly status

Sequence Verified

  • <bbpart>I13521</bbpart> - R0040.B0034.E1010.B0015
  • <bbpart>I13573</bbpart> - R0040.hairpin.B0034.E1010.B0015
  • <bbpart>I13575</bbpart> - R0050.hairpin.B0034.E1010.B0015
  • <bbpart>I13574</bbpart> - R0050.B0034.E1010.B0015
  • We also have a version of <bbpart>I13572</bbpart> with AC inserted in position 3 of the hairpin (otherwise perfect).

Failed

  • <bbpart>I13572</bbpart> - R0040.hairpin.B0034.E1010
    • AC insert in position 3 of the hairpin
  • <bbpart>I13576</bbpart> - R0050.hairpin.B0034.E1010
    • Failed at ligation/transformation step (no colonies).
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