DNA blot (Southern)

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The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
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== Lab-specific DNA blotting protocols ==
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* [[Oneill_Lab:Southern_Blot]]
== See also ==
== See also ==
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* [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video]
* [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video]
* [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson]
* [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson]
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* [http://www.dmd.nl/protocol/DNAhybridization_ALG005.html detailed DNA blot protocol from Leiden University]
* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]
* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]

Revision as of 10:12, 16 April 2009

The DNA blot or Southern blot is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a restriction endonuclease. Fragments are then separated by size using agarose gel electrophoresis. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.

Lab-specific DNA blotting protocols

See also

External links

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